41. Trevino AE#, Müller F#, Andersen J#, Sundaram L#, Kathiria A, Shcherbina A, Farh K, Chang HY, Pașca AM, Kundaje A,
Pașca SP*, Greenleaf WJ*.
Chromatin and gene-regulatory dynamics of the developing human cerebral cortex at single-cell resolution.
BioRxiv: doi.org/10.1101/2020.12.29.424636 (2020)
Pașca SP*, Greenleaf WJ*.
Chromatin and gene-regulatory dynamics of the developing human cerebral cortex at single-cell resolution.
BioRxiv: doi.org/10.1101/2020.12.29.424636 (2020)
40. Kelley K, Pașca SP*.
Scrutinizing disease states and regulation of human microglia.
Nature Genetics (in press)
Scrutinizing disease states and regulation of human microglia.
Nature Genetics (in press)
39. Agoglia RM, Sun D, Birey F, Yoon SJ, Miura Y, Sabatini K, Pașca SP* & Fraser HB*.
Primate cell fusion disentangles gene regulatory divergence in neurodevelopment.
Nature (advanced online publication)
Primate cell fusion disentangles gene regulatory divergence in neurodevelopment.
Nature (advanced online publication)
38. Gordon A, Yoon S-Y, Tran SS, Makinson CD, Park JY, Andersen H, Valencia AM, Horvath S, Xiao X, Huguenard JR,
Paşca SP*, Geschwind DH*.
Long-term maturation of human cortical organoids matches key early postnatal transitions
Nature Neuroscience (advanced online publication).
Website: GECO
Paşca SP*, Geschwind DH*.
Long-term maturation of human cortical organoids matches key early postnatal transitions
Nature Neuroscience (advanced online publication).
Website: GECO
37. Andersen J, Revah O, Miura Y, Thom N, Amin ND, Kelley KW, Singh M, Chen X, Thete MV, Walczak EM, Vogel H, Fan HC, and Paşca SP*.
Generation of functional human 3D cortico-motor assembloids.
Cell 183 (7): P1913-1929 (2020). Cover article. [PDF]
[Stanford press release] [NYSCF]
Generation of functional human 3D cortico-motor assembloids.
Cell 183 (7): P1913-1929 (2020). Cover article. [PDF]
[Stanford press release] [NYSCF]
36. Miura Y, Li M-Y, Birey Y, Ikeda K, Revah O, Thete MV, Park J-Y, Puno A, Lee SH, Porteus MH, and Paşca SP*.
Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells.
Nature Biotechnology 38:1421-1430 (2020). [PDF]
Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells.
Nature Biotechnology 38:1421-1430 (2020). [PDF]
35. Khan T#, Revah O#, Gordon A#, Yoon S-Y, Krawisz A, Goold C, Sun Y, Kim C-H, Tian Y, Li M-Y, Schaepe J, Ikeda K, Neal AD, Sakai N, Yazawa M, Kushan L, Nishino S, Porteus M, Rapoport J, Bernstein J, O'Hara R, Bearden C, Hallmayer J, Huguenard J, Geschwind DH, Dolmetsch RE, and Paşca SP*.
Neuronal defects in a human cellular model of 22q11.2 deletion syndrome.
Nature Medicine (advanced online publication).
[Stanford press release] [NIH press release] [Spectrum] [CIRM]
Neuronal defects in a human cellular model of 22q11.2 deletion syndrome.
Nature Medicine (advanced online publication).
[Stanford press release] [NIH press release] [Spectrum] [CIRM]
34. Paşca SP, Veenstra-VanderWeele J, McPartland JC.
Research and training in autism spectrum disorder to catalyze the next genomic and neuroscience revolutions.
Molecular Psychiatry (advance online publication). [PDF]
33. Liu J, Kim YS, Richardson C, Tom A, Ramakrishnan C, Birey F, Katsumata T, Chen S, Wang C, Wang X, Joubert L-M, Jiang Y, Wang H, Fenno LE, Tok JB-H, Paşca SP, Shen K, Bao Z*, and Deisseroth K*.
Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals.
Science 367 (6484): 1372-76 (2020). [PDF]
32. Trevino A#, Sinnott-Armstrong NA#, Andersen J#, Yoon S-J, Huber N, Pritchard J, Howard CY, Greenleaf WJ* & Paşca SP*.
Dynamic changes in chromatin states in a model of human forebrain development.
Science 367: 404 (2020) [PDF].
Website: http://brainchromatin.stanford.edu/
Research and training in autism spectrum disorder to catalyze the next genomic and neuroscience revolutions.
Molecular Psychiatry (advance online publication). [PDF]
33. Liu J, Kim YS, Richardson C, Tom A, Ramakrishnan C, Birey F, Katsumata T, Chen S, Wang C, Wang X, Joubert L-M, Jiang Y, Wang H, Fenno LE, Tok JB-H, Paşca SP, Shen K, Bao Z*, and Deisseroth K*.
Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals.
Science 367 (6484): 1372-76 (2020). [PDF]
32. Trevino A#, Sinnott-Armstrong NA#, Andersen J#, Yoon S-J, Huber N, Pritchard J, Howard CY, Greenleaf WJ* & Paşca SP*.
Dynamic changes in chromatin states in a model of human forebrain development.
Science 367: 404 (2020) [PDF].
Website: http://brainchromatin.stanford.edu/
31. Marton RM and Pașca SP*.
Organoid and assembloid technologies for investigating cellular crosstalk in human brain development and disease.
Trends Cell Biology [Cover article]. [PDF]
31. Marton RM and Pașca SP*.
Organoid and assembloid technologies for investigating cellular crosstalk in human brain development and disease.
Trends Cell Biology [Cover article]. [PDF]
30. Panagiotakos G, Haveles C, Arjun A, Petrova R, Rana A, Portmann T, Paşca SP, Palmer SP, Dolmetsch RE.
Aberrant Calcium Channel Splicing Drives Defects in Cortical Differentiation in Timothy Syndrome.
Elife 8:e51037, 2019. [PDF]
29. Pașca SP.
The hidden biology of the human brain.
Nature Medicine 25: 1655 (2019). [PDF]
Aberrant Calcium Channel Splicing Drives Defects in Cortical Differentiation in Timothy Syndrome.
Elife 8:e51037, 2019. [PDF]
29. Pașca SP.
The hidden biology of the human brain.
Nature Medicine 25: 1655 (2019). [PDF]
28. Sanders SJ, Sahin M, Hostyk J, Thurm A, Jacquemont S, Avillach P, Douard E, Martin CL, Modi ME, Moreno-De-Luca A, Raznahan A, Anticevic A, Dolmetsch R, Feng G, Geschwind DH, Glahn DC, Goldstein DB, Ledbetter DH, Mulle JG, Paşca SP, Samaco R, Sebat J, Pariser A, Lehner T, Gur RE, Bearden CE.
A framework for the investigation of rare genetic disorders in neuropsychiatry.
Nature Medicine 25: 1477–1487 (2019). [PDF]
A framework for the investigation of rare genetic disorders in neuropsychiatry.
Nature Medicine 25: 1477–1487 (2019). [PDF]
27. Li TL, Wang Z, You H, Ong Q, Varanasi VJ, Dong M, Lu B, Paşca SP, Cui B.
Engineering a Genetically Encoded Magnetic Protein Crystal.
Nano Letters 19(10), 6955-6963 (2019). [PDF]
Engineering a Genetically Encoded Magnetic Protein Crystal.
Nano Letters 19(10), 6955-6963 (2019). [PDF]
26. Kratochvil M, Seymour A, Li TL, Paşca SP, Kuo C, Heilshorn S.
Engineered materials for organoid systems.
Nature Materials Reviews 4: 606-622 (2019). [PDF]
Engineered materials for organoid systems.
Nature Materials Reviews 4: 606-622 (2019). [PDF]
25. Paşca AM, Park J-Y, Shin H-W, Qi Q, Revah O, Krasnoff R, O'Hara R, Willsey JA, Palmer TD and Paşca SP*.
Human 3D cellular model of hypoxic brain injury of prematurity.
Nature Medicine 25:784-791 (2019) [Cover Article] [PDF]
[Press Release] [Scope] [US News & World Report] [CIRM]
24. Arlotta P* and Paşca SP*.
Cell diversity in the human cerebral cortex: from the embryo to brain organoids.
Current Opinion in Neurobiology 30 (56):194-198 (2019). [PDF]
Cell diversity in the human cerebral cortex: from the embryo to brain organoids.
Current Opinion in Neurobiology 30 (56):194-198 (2019). [PDF]
23. Geraghty AC, Gibson EM, Ghanem RA, Greene JJ, Ocampo A, Goldstein AK., Ni L, Yang T, Marton RM, Paşca SP, Greenberg ME, Longo FM and Monje, M.
Loss of Adaptive Myelination Contributes to Methotrexate Chemotherapy-Related Cognitive Impairment.
Neuron 103(2): 250-265 (2019).
Loss of Adaptive Myelination Contributes to Methotrexate Chemotherapy-Related Cognitive Impairment.
Neuron 103(2): 250-265 (2019).
22. Miura Y and Paşca SP*.
Polarizing brain organoids.
Nature Biotechnology 37: 377-78 (2019).
Polarizing brain organoids.
Nature Biotechnology 37: 377-78 (2019).
21. Marton RM, Miura Y, Sloan SA, Li Q, Revah O, Levy RJ, Huguenard JR, and Paşca SP*.
Differentiation and maturation of oligodendrocytes in human three-dimensional neural cultures.
Nature Neuroscience 22(3): 485-491 (2019). [PDF]
[Press release]
Differentiation and maturation of oligodendrocytes in human three-dimensional neural cultures.
Nature Neuroscience 22(3): 485-491 (2019). [PDF]
[Press release]
20. Paşca SP*.
Assembling human brain organoids.
Science 363:126-127 (2019). [PDF]
Assembling human brain organoids.
Science 363:126-127 (2019). [PDF]
19. Yoon SJ, Elahi LS, Pasca AM, Marton RM, Gordon A, Revah O, Miura Y, Walczak EM, Holdgate G, Fan CH, Huguenard JR, Geschwind DH and Paşca SP*.
Reliability of human cortical organoid generation.
Nature Methods 16:75-78 (2018). [PDF]
18. Amin N and Paşca SP*.
Building models of brain disorders with three-dimensional organoids.
Neuron 100:389-405, 2018. [PDF]
[30 years anniversary issue].
17 Andersen J and Paşca SP*.
Absent forebrain replaced by embryonic stem cells.
Nature 563 (7729):44-45 (2018).
Absent forebrain replaced by embryonic stem cells.
Nature 563 (7729):44-45 (2018).
16. Sloan SA (co-first), Andersen JA (co-first), Paşca AM (co-first), Birey F (co-first), and Paşca SP*.
Generation and assembly of human brain region-specific three-dimensional cultures.
Nature Protocols, 13: 2062-2085, 2018 [Cover Article]. [PDF]
15. Paşca SP*.
Building three-dimensional human brain organoids.
Nature Neuroscience, 2018.
doi:10.1038/s41593-018-0107-3
14. Farahany NA, Greely HT, Hyman S, Koch C, Grady C, Pașca SP, Sestan N, Arlotta P, Bernat JL, Ting J, Lunshof JE, Iyer EPR, Hyun I, Capestany BH, Church GM, Huang H, Hongjun S.
The ethics of experimenting with human brain tissue
Nature 556: 429–432, 2018.
The ethics of experimenting with human brain tissue
Nature 556: 429–432, 2018.
13. Marton RM and Paşca SP*.
A human cellular model of amyotrophic lateral sclerosis.
Nature Medicine 24(3): 256-257, 2018
A human cellular model of amyotrophic lateral sclerosis.
Nature Medicine 24(3): 256-257, 2018
11. Sloan SA, Darmanis S, Huber N, Khan TA, Birey F, Caneda C, Reimer R, Quake SR, Barres BA*, and Paşca SP*.
Human Astrocyte Maturation Captured in 3D Cortical Spheroids Derived from Human Pluripotent Stem Cells.
Neuron, 95: 779-790, 2017.
[Featured Article] [Preview] [NIH Press Release] [Scope]
Image: "Persistence of Astrocytes" by Mesa Schumacher
11. Sloan SA, Darmanis S, Huber N, Khan TA, Birey F, Caneda C, Reimer R, Quake SR, Barres BA*, and Paşca SP*.
Human Astrocyte Maturation Captured in 3D Cortical Spheroids Derived from Human Pluripotent Stem Cells.
Neuron, 95: 779-790, 2017.
[Featured Article] [Preview] [NIH Press Release] [Scope]
Image: "Persistence of Astrocytes" by Mesa Schumacher
9. Madelaine R, Sloan SA, Huber N, Notwell JH, Leung LC, Skariah G, Hulluin C, Paşca SP, Bejerano G, Krasnow MA, Barres BA, Mourrain P.
MicroRNA-9 Couples Brain Neurogenesis and Angiogenesis.
Cell Reports 20 (7), 1533-1542, 2017.
8. Birey F (co-first), Andersen J (co-first), Makinson C (co-first), Islam S, Wei W, Huber N, Fan CH, Metzler K, Panagiotakos G, Thom N, O’Rourke NA, Steinmetz LM, Bernstein JA, Hallmayer J, Huguenard JR, and Paşca SP*.
Assembly of functionally integrated human forebrain spheroids.
Nature (Article), 545: 54-59, 2017.
[Stanford Press release] [NIH Press release] [MQ] [NIH Video] [Scope Blog]
[F1000] [NIH Highlights of 2017] [NARSAD/BBRF Top Advances of 2017]
Assembly of functionally integrated human forebrain spheroids.
Nature (Article), 545: 54-59, 2017.
[Stanford Press release] [NIH Press release] [MQ] [NIH Video] [Scope Blog]
[F1000] [NIH Highlights of 2017] [NARSAD/BBRF Top Advances of 2017]
7. Can Y, Hjorth M, Chen H, Birey F, Leal-Ortiz S, Han C, Santiago JG, Paşca SP, Wu J, Melosh NA.
Non-destructive nanostraw intracellular sampling for longitudinal cell monitoring.
Proceedings of the National Academy of Sciences, 114(10): E1866-74 (2017).
[Press release] [Cozzarelli Prize]
6. Marton RM and Paşca SP*.
Neural differentiation in the third dimension: making a human midbrain.
Cell Stem Cells, 19(2):145-6, 2016.
Neural differentiation in the third dimension: making a human midbrain.
Cell Stem Cells, 19(2):145-6, 2016.
5. Sun Y, Paşca SP, Portmann T, Goold C, Worringer KA, Guan W, Chan K, Gai H, Vogt D, Chen Y-JJ, Mao R, Chan K, Rubenstein J, Madison DV, Hallmayer J, Froehlich-Santino WM, Bernstein J, and Dolmetsch RE.
A deleterious NaV1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet Syndrome patients.
eLife, 5: e13073, 2016.
4. Paşca SP*.
Personalized Human Cortical Spheroids.
American Journal of Psychiatry, 173(4): 332-3, 2016.
Personalized Human Cortical Spheroids.
American Journal of Psychiatry, 173(4): 332-3, 2016.
3. Deverman BE, Pravdo PL, Simpson BP, Kumar SR, Chan KY, Banerjee A, Wu W-L, Yang B, Huber N, Paşca SP, Gradinaru V.
Cre-dependent capsid selection yields AAVs for global gene transfer to the adult brain.
Nature Biotechnology, 34(2): 204-9, 2016 .
2. Paşca AM (co-first), Sloan S (co-first), Clarke LE, Tian Y, Makinson C, Huber N, Kim C-H, Park J-Y, O’Rourke NA, Nguyen K, Smith SJ, Huguenard J, Geschwind DH, Barres BA, and Paşca SP*.
Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D cultures.
Nature Methods, 12: 671-678, 2015.
[Press release] [Blog][NIH Press Release] [SFARI] [F1000] [Key Advances in hiPSC Modeling]
1. Paşca SP*, Panagiotakos G, Dolmetsch RE.
Generating human neurons in vitro and using them to understand neuropsychiatric disease.
Annual Review of Neuroscience, 37: 479-501; 2014.
Tian Y, Voineagu I, Paşca SP, Won H, Chandran V, Horvath S, Dolmetsch RE, Geschwind DH.
Alteration in basal and depolarization induced transcriptional network in iPSC derived neurons from Timothy syndrome.
Genome Medicine, 6:75, 2014.
[Editor's pick]
Esposito G, and Paşca SP.*
Motor abnormalities as a putative endophenotype for autism spectrum disorders.
Frontiers in Neuroscience 7: 43, 2013;
Gomez-Ospina N, Panagiotakos G, Portmann T, Paşca SP, Rabah D, Budzillo A, Kinet J-P, Dolmetsch RE.
A promoter in the coding region of the calcium channel gene CACNA1C generates the transcription factor CCAT.
PLoS ONE 8(4): e60526, 2013.
Alteration in basal and depolarization induced transcriptional network in iPSC derived neurons from Timothy syndrome.
Genome Medicine, 6:75, 2014.
[Editor's pick]
Esposito G, and Paşca SP.*
Motor abnormalities as a putative endophenotype for autism spectrum disorders.
Frontiers in Neuroscience 7: 43, 2013;
Gomez-Ospina N, Panagiotakos G, Portmann T, Paşca SP, Rabah D, Budzillo A, Kinet J-P, Dolmetsch RE.
A promoter in the coding region of the calcium channel gene CACNA1C generates the transcription factor CCAT.
PLoS ONE 8(4): e60526, 2013.
Krey JK, Paşca SP, Shcheglovitov O, Yazawa M, Schwemberger R, Rasmusson R, Dolmetsch RE.
Timothy syndrome is associated with activity-dependent dendritic retraction in rodent and human neurons.
Nature Neuroscience 16(12): 201-09, 2013.
[Featured in Science Translational– Editor’s Choice]
Paşca SP, Portmann T, Yazawa M, Voineagu I, Pasca AM, Shcheglovitov O, Cord B, Palmer TD, Chikahisa S, Seiji N, Bernstein JA, Hallmayer J, Geschwind DH, and Dolmetsch RE.
Using iPSC-derived neurons to uncover cellular phenotypes associated with Timothy syndrome.
Nature Medicine, 17(12): 1657-62, 2011. (Cover article)
[News & Views by Huttner & Rakic]; [Highlighted in Nature Neuroscience Reviews, Cell, JAMA]; [evaluated by F1000]; [Best 2012 Neuroscience article published at Stanford University]; [among NIMH top 10 advances of 2011] [SFARI top 10 papers of 2011] [selected for NatureOutlook Autism Collection].
Paşca SP, Singer W, and Nikolic D.
Surround modulation of neuronal responses in V1 is as stable over time as responses to direct stimulation of receptive fields.
Cortex, 46(9): 199-203, 2010.
Paşca SP, Dronca E, Nemes B, Kaucsár T, Endreffy E, Iftene F, Benga I, Cornean R, and Dronca M.
Paraoxonase 1 activities and polymorphisms in Autism Spectrum Disorders.
Journal of Cellular and Molecular Medicine, 14(3): 600-7, 2010.
Paşca SP, Dronca E, Kaucsár T, Craciun EC, Endreffy E, Ferencz BK, Iftene F, Benga I, Cornean R, Banerjee R, and Dronca M.
One Carbon Metabolism Disturbances and the C677T MTHFR Gene Polymorphism in Children with Autism Spectrum Disorders.
Journal of Cellular and Molecular Medicine, 14(10): 4229-4238, 2009.
Paşca SP and Nesse RM.
Vomiting is not an adaption for glaucoma (and Darwinian medicine is difficult).
Medical Hypotheses, 71(3): 472-3, 2008.
Miu AC, Heilman RM, Paşca SP, Stefan CA, Spanu F., Vasiu R., Olteanu A.I., and Miclea M.
Behavioral effects of enriched environment on adult rats with corpus callosum transection.
Behavioral Brain Research, 172(1): 134-144, 2006.
Paşca SP, Nemes B, Vlase L, Gagyi CE, Dronca E, Miu AC and Dronca M.
High levels of homocysteine and low serum paraoxonase 1 arylesterase activity in children with autism.
Life Sciences, 78(19): 2244-2248, 2006.
Miu AC, Paşca SP and Miclea M.
The corpus callosum and the power of complementary paradigms in developmental cognitive neuroscience.
Cognition, Brain, Behavior, 9: 423-450, 2005.
Surround modulation of neuronal responses in V1 is as stable over time as responses to direct stimulation of receptive fields.
Cortex, 46(9): 199-203, 2010.
Paşca SP, Dronca E, Nemes B, Kaucsár T, Endreffy E, Iftene F, Benga I, Cornean R, and Dronca M.
Paraoxonase 1 activities and polymorphisms in Autism Spectrum Disorders.
Journal of Cellular and Molecular Medicine, 14(3): 600-7, 2010.
Paşca SP, Dronca E, Kaucsár T, Craciun EC, Endreffy E, Ferencz BK, Iftene F, Benga I, Cornean R, Banerjee R, and Dronca M.
One Carbon Metabolism Disturbances and the C677T MTHFR Gene Polymorphism in Children with Autism Spectrum Disorders.
Journal of Cellular and Molecular Medicine, 14(10): 4229-4238, 2009.
Paşca SP and Nesse RM.
Vomiting is not an adaption for glaucoma (and Darwinian medicine is difficult).
Medical Hypotheses, 71(3): 472-3, 2008.
Miu AC, Heilman RM, Paşca SP, Stefan CA, Spanu F., Vasiu R., Olteanu A.I., and Miclea M.
Behavioral effects of enriched environment on adult rats with corpus callosum transection.
Behavioral Brain Research, 172(1): 134-144, 2006.
Paşca SP, Nemes B, Vlase L, Gagyi CE, Dronca E, Miu AC and Dronca M.
High levels of homocysteine and low serum paraoxonase 1 arylesterase activity in children with autism.
Life Sciences, 78(19): 2244-2248, 2006.
Miu AC, Paşca SP and Miclea M.
The corpus callosum and the power of complementary paradigms in developmental cognitive neuroscience.
Cognition, Brain, Behavior, 9: 423-450, 2005.
